MORAN LABORATORY APHID CLONES
All A. pisum clones are available to send out if indicated in the third column below; this includes all featured in publications that are still being maintained as live cultures. Every colony descends from one female in the lab; colonies are kept on Vicia faba seedlings at constant 20oC under long day conditions (16L:8D). To prevent contamination, several safeguards are included in the protocol for colony maintenance, and samples of each colony are checked every spring using AFLP markers that distinguish clones (see Oliver et al. 2003).
For more information or to request insects, please contact Nancy Moran by email: nmoran@email.arizona.edu.
Publications mentioned here (linked to PubMed abstracts):
Sandström, J. P., J. A. Russell, J. P. White, and N. A. Moran. 2001. Independent origins and horizontal transfer of bacterial symbionts of aphids. Molecular Ecology 10: 217-228
Oliver, K., J. Russell, N. Moran, and M. Hunter. 2003. Facultative bacterial symbionts in aphids confer resistance to parasitic wasps. Proceedings of the National Academy of Sciences USA 100:1803-1807
Moran, N. A., J. A. Russell, R. Koga, and T. Fukatsu. 2005. Evolutionary relationships of three new species of Enterobacteriaceae living as symbionts of aphids and other insects. Applied and Environmental Microbiology 71(6): 3302-10
Oliver, K. M., N. A. Moran, and M. S. Hunter. 2005. Variation in resistance to parasitism in aphids is due to symbionts and not host genotype. Proceedings of the National Academy of Sciences USA 102(36): 12795-800
Russell, J. A. and N. A. Moran. 2005. Horizontal transfer of bacterial symbionts: heritability and fitness effects in a novel aphid host. Applied and Environmental Microbiology 71(12): 7987-94
Russell, J. A. and N. A. Moran. 2006. Costs and benefits of symbiont infection in aphids: variation among symbionts and across temperatures. Proceedings of the Royal Society B 273(1586): 603-610
Dunbar, H.E., Wilson, A.C., Ferguson, N.R., Moran, N.A. 2007. Aphid thermal tolerance is governed by a point mutation in bacterial symbionts. PLoS Biology 5(5): e96
Download this table as an MS Excel file.
last updated: 11/26/07 by NM
| Species | Clone Name | Available to Send Out | Color | Collected/ Estab. Month | Coll. Day | Collected/ Estab. Year | Collection Loc. | Collector | Collection Host plant | Collected with Secondary Symbionts | Short ibpA Promoter (Dunbar et al 2006) | Derived from Clone | Current Secondary Symbionts | Inoculation or Cure | Ability to Make Sexuals | Wingedness of Males | Wing Genotype | Color Genotype (R=red, dominant) | Other Culture Conditions | Publications (first author, journal, year) | Misc. | Clone Name | Species |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A. pisum | 2a | y | green | May | 2000 | Tompkins Co., NY | Jacob Russell | alfalfa | R. insecticola | long | R. insecticola | yes | both | w/wl | rr | Sandstrom MolEcol 2001; Moran AEM 2007 | 2a | A. pisum | |||||
| A. pisum | 7a | y | pink | May | 2000 | Cayuga Co., NY | Jacob Russell | Medicago sativa | none | short | none | yes | Sandstrom MolEcol 2001 | 7a | A. pisum | ||||||||
| A. pisum | 8-2b | y | pink | July | 2000 | Cayuga Co., NY | Jacob Russell | Medicago sativa | H. defensa | long | H. defensa | yes | wingless | wl/wl | Rr | Oliver PNAS 2003; Russell AEM 2005 | 8-2b | A. pisum | |||||
| A. pisum | 7-2-1 | y | green | August | 2001 | Cayuga Co., NY | Jacob Russell | Medicago sativa | none | short | none | yes | w/wl? | rr | Russell AEM 2005 | 7-2-1 | A. pisum | ||||||
| A. pisum | 9-2-1 | y | green | August | 2001 | Cayuga Co., NY | Jacob Russell | Medicago sativa | S. symbiotica | long | S. symbiotica | yes | wingless | wl/wl | rr | Russell PRSLB 2006 | 9-2-1 | A. pisum | |||||
| A. pisum | 8-10-1 | y | green | August | 2001 | Cayuga Co., NY | Jacob Russell | Medicago sativa | R. insecticola | long | R. insecticola | yes | Russell AEM 2005 | 8-10-1 | A. pisum | ||||||||
| A. pisum | 2BB | y | green | June | 26 | 1999 | Madison, WI arboretum | Nancy Moran | Medicago lupulina | S. symbiotica | long | S. symbiotica | yes | Sandstrom MolEcol 2001 | 2BB | A. pisum | |||||||
| A. pisum | Tucson | y | green | May | 20 | 1999 | Tucson, AZ, west University area | Nancy Moran | Vicia faba | S. symbiotica | long | S. symbiotica | no | Sandstrom MolEcol 2001; Russell PRSLB 2006; Moran AEM 2007 | Tucson | A. pisum | |||||||
| A. pisum | 5A | y | pink | June | 26 | 1999 | Madison, WI arboretum | Nancy Moran | Medicago lupulina | none | short | none | yes | winged | w/w | Rr | Sandstrom MolEcol 2001; Dunbar PLoS Biology 2007 | This mutated from an ibpA promoter with an "11" spacer to one with a "10" Details are in Dunbar et al. 2007 | 5A | A. pisum | |||
| A. pisum | 5A-T | y | pink | July | 9 | 2001 | N/A | 5A | H. defensa | inoculation | yes | winged | Oliver PNAS 2003, 2005; Russell AEM 2005 | inoculated by JR | 5A-T | A. pisum | |||||||
| A. pisum | 5A-R | y | pink | September | 22 | 2000 | N/A | 5A | S. symbiotica | inoculation | yes | winged | Russell PRSLB 2006 | inoculated by JR | 5A-R | A. pisum | |||||||
| A. pisum | 5A-U | y | pink | January | 19 | 2001 | N/A | 5A | R. insecticola | inoculation | yes | winged | Russell AEM 2005 | inoculated by JR | 5A-U | A. pisum | |||||||
| A. pisum | A2C | y | green | May | 22 | 2003 | Logan, Utah | Kerry Oliver | H. defensa | long | H. defensa | yes | winged | w/wl | rr | Oliver PNAS 2003 | A2C | A. pisum | |||||
| A. pisum | Tuc7 | y | green | 2007 | Tucson, AZ, Campbell Ave farm | Nancy Moran | Medicago sativa | long | S. symbiotica | no | na | Tuc7 | A. pisum | ||||||||||
| A. pisum | A2A | y | green | May | 22 | 2003 | Logan, Utah | Kerry Oliver | Medicago sativa | long | none | yes | Oliver PNAS 2005 | A2A | A. pisum | ||||||||
| A. pisum | Tucson cure | n | green | 2006 | N/A | Vicia faba in lab | long | Tucson | none | cure through repeated heat treatments | no | Tucson cure | A. pisum | ||||||||||
| A. pisum | CAG27 | n | green | March | 31 | 2007 | Walnut Creek, CA | Nancy Moran | Medicago lupulina | ? | S. symbiotica | no | na | CAG27 | A. pisum | ||||||||
| A. pisum | SC7 | n | green | March | 17 | 2007 | Charleston, SC | Nancy Moran | Medicago lupulina | ? | S. symbiotica | na | SC7 | A. pisum | |||||||||
| A. pisum | 5A11R yellow | n | yellow | spring | 2007 | N/A | Helen Dunbar | Vicia faba in lab | This is a mutant that switched from pink to yellow color | long | 5AR | S. symbiotica | yes | 5A11R yellow | A. pisum | ||||||||
| A. pisum | 5A10 yellow | n | yellow | July | 27 | 2007 | N/A | Kim Hammond | Vicia faba in lab | This is a mutant that switched from pink to yellow color | short | 5A | yes | 5A10 yellow | A. pisum | ||||||||
| A. pisum | Ars5A11 | n | 2007 | N/A | Eva Novakova? | Vicia faba in lab | long | 5A | Arsenophonus | inoculation | yes | Russell AEM 2005 | Ars5A11 | A. pisum | |||||||||
| A. pisum | 47-1/2 #18 | n | pink | from Helen's ISTS experiment | (double secondaries) | from cross, paternal transfer | 47-1/2 #18 | A. pisum | |||||||||||||||
| A. pisum | 47-1/2 #5 | n | pink | from Helen's ISTS experiment | (double secondaries) | 47-1/2 #5 | A. pisum | ||||||||||||||||
| A. pisum | 47 #5 | n | pink | from Helen's ISTS experiment | (double secondaries) | 47 #5 | A. pisum | ||||||||||||||||
| A. pisum | 47-1/2 #2 | n | green | from Helen's ISTS experiment | (double secondaries) | 47-1/2 #2 | A. pisum | ||||||||||||||||
| A. pisum | 47 #2 | n | green | from Helen's ISTS experiment | (double secondaries) | 47 #2 | A. pisum | ||||||||||||||||
| A. nerii (MO1) | n | Tucson, AZ | Nancy Moran | (milkweed) | A. nerii (MO1) | ||||||||||||||||||
| S. avenae | n | Tucson? | Jennifer Wilcox | barley | S. avenae | ||||||||||||||||||
| S. graminum | n | alfalfa | S. graminum | ||||||||||||||||||||
| A. kondoi | n | March | 21 | 2007 | Tucson, AZ | Kim Hammond | Medicago arborea | (double secondaries) | alfalfa | A. kondoi | |||||||||||||
| A. craccivora | n | Alex or Patrick? | A. craccivora |